EBF-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56773

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EBF-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MFGIQESIQRSGSSMKEEPLGSGMN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit EBF-1 Antibody - BSA Free (NBP2-56773) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EBF-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EBF-1 Antibody [NBP2-56773]

Immunocytochemistry/ Immunofluorescence: EBF-1 Antibody [NBP2-56773]

Immunocytochemistry/Immunofluorescence: EBF-1 Antibody [NBP2-56773] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.
EBF-1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: EBF-1 Antibody - BSA Free [NBP2-56773]

Chromatin Immunoprecipitation-exo-Seq: EBF-1 Antibody - BSA Free [NBP2-56773]

ChIP-Exo-Seq composite graph for Anti-EBF1 (NBP2-56773) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for EBF-1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBF-1

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (Bysimilarity)

Long Name

Early B-cell Factor 1

Alternate Names

COE1, EBF1, OE-1, OLF1

Gene Symbol

EBF1

Additional EBF-1 Products

Product Documents for EBF-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EBF-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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