ECH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83215

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ECH1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ECH1 Antibody - BSA Free

Immunohistochemistry: ECH1 Antibody [NBP1-83215]

Immunohistochemistry: ECH1 Antibody [NBP1-83215]

Immunohistochemistry: ECH1 Antibody [NBP1-83215] - Staining of mouse olfactory bulb shows positivity in the mitral cell layer.
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215] - Staining of human liver shows strong cytoplasmic positivity in a granular pattern in hepatocytes.
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215] - Staining of human cerebral cortex shows strong cytoplasmic immunoreactivity in neurons.
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-83215] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cell bodies.
Immunohistochemistry: ECH1 Antibody [NBP1-83215]

Immunohistochemistry: ECH1 Antibody [NBP1-83215]

Immunohistochemistry: ECH1 Antibody [NBP1-83215] - Staining of mouse caudate putamen shows positivity in neurons and blood vessels.
Immunohistochemistry: ECH1 Antibody [NBP1-83215]

Immunohistochemistry: ECH1 Antibody [NBP1-83215]

Immunohistochemistry: ECH1 Antibody [NBP1-83215] - Staining of mouse hippocampus shows immunoreactivity in the CA1 region.
ECH1 Antibody - BSA Free Western Blot: ECH1 Antibody - BSA Free [NBP1-83215]

Western Blot: ECH1 Antibody - BSA Free [NBP1-83215]

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
ECH1 Antibody - BSA Free Western Blot: ECH1 Antibody - BSA Free [NBP1-83215]

Western Blot: ECH1 Antibody - BSA Free [NBP1-83215]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells))
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells))

Applications for ECH1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECH1

ECH1 encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Alternate Names

delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, delta3,5-delta2,4-dienoyl-CoA isomerase, dienoyl-CoA isomerase, EC 5.3.3.-, enoyl CoA hydratase 1, peroxisomal, enoyl Coenzyme A hydratase 1, peroxisomal, HPXEL, peroxisomal enoyl-CoA hydratase 1

Gene Symbol

ECH1

Additional ECH1 Products

Product Documents for ECH1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ECH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ECH1 Antibody - BSA Free

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Protocols

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