ECRG4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84911

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ECRG4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KREAPVPTKTKVAVDENKAKEFLGSLKRQKRQLWDRTRPEVQQWYQQFLYMGFDEAKFEDDITYWLNRDRNGHEYYGDYYQRHYDEDSAIGPRSPYGFRHGASVNYD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%). Rat reactivity reported in scientific literature (PMID: 26150152).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ECRG4 Antibody - BSA Free

Western Blot: ECRG4 Antibody [NBP1-84911]

Western Blot: ECRG4 Antibody [NBP1-84911]

Western Blot: ECRG4 Antibody [NBP1-84911] - Analysis in control (vector only transfected HEK293T lysate) and C2orf40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911]

Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human placenta shows moderate positivity in plasma.

Applications for ECRG4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECRG4

ECRG4, also known as C2orf40 or Chromosome 2 Open Reading Frame 40, is a secreted hormone that may stimulate senescence of neural precursor cells and oligodendrocytes. Current research on ECRG4is being performed in relation to several diseases and disorders including esophageal cancer, esophagitis and breast cancer. ECRG4 has also been shown to interact with p53.

Alternate Names

chromosome 2 open reading frame 40, ECRG4augurin, esophageal cancer related gene 4 protein, Esophageal cancer-related gene 4 protein

Gene Symbol

ECRG4

Additional ECRG4 Products

Product Documents for ECRG4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ECRG4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ECRG4 Antibody - BSA Free

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Protocols

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