Recombinant Human EGF Protein

Novus Biologicals | Catalog # NBP2-34952

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P01133

Applications

Bioactivity, SDS-PAGE
View all EGF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to EGF Source: E. coli

Uniprot ID: P01133

Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of EGF as determined by LAL method.

Predicted Molecular Mass

6.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBP2-34952 in the following applications:

Scientific Data Images for Recombinant Human EGF Protein

SDS-PAGE: Recombinant Human EGF Protein [NBP2-34952]

SDS-PAGE: Recombinant Human EGF Protein [NBP2-34952]

SDS-Page: Human EGF Protein [NBP2-34952]

Formulation, Preparation, and Storage

NBP2-34952
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF

EGF R (ErbB1) is a receptor tyrosine kinase that is widely expressed on epithelial cells and is required for epithelial cell development and proliferation. EGF R binds the EGF family ligands: EGF, Amphiregulin, TGF-alpha, Betacellulin, Epiregulin, HB-EGF, and Epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, ErbB3, or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Alterations in the structure, expression, and signaling of EGF R are involved in the development and metastasis of a wide variety of cancers.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Gene Symbol

EGF

UniProt

P01133

Additional EGF Products

Product Documents for Recombinant Human EGF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Human EGF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human EGF Protein

Customer Reviews for Recombinant Human EGF Protein (1)

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  • Human EGF Protein
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 01/04/2017
    SDS PAGE gel with Coomassie blue staining showing detection of Human EGF protein (NBP2-34952-100 ug ) (5 ug).
    Recombinant Human EGF Protein NBP2-34952

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