A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to EGF, including 3 intramolecular disulfide-bonds Source: E. coli

Uniprot ID: P01132

Amino Acid Sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of EGF as determined by LAL method.

Predicted Molecular Mass

6.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse EGF Protein

SDS-PAGE: Recombinant Mouse EGF Protein [NBP2-35176]

SDS-Page: Recombinant Mouse EGF Protein [NBP2-35176]

Formulation, Preparation, and Storage

NBP2-35176

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: EGF

Epidermal growth factor (EGF) is the founding member of the EGF family of growth factors. Mature EGF is generated by proteolytic release of a juxtamembrane domain within the transmembrane EGF precursor. It is present in a wide variety of body fluids. EGF binds the receptor ErbB1 and induces signaling through ErbB1 homodimers or heterodimers with ErbB2. It plays a key role in epithelial morphogenesis, angiogenesis, and tumorigenesis.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Gene Symbol

EGF

UniProt

P01132

Additional EGF Products

Product Documents for Recombinant Mouse EGF Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Mouse EGF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.