EGTA

Tocris Bioscience | Catalog # 2807

Calcium chelating agent
Tocris Bioscience

Key Product Details

Description

Calcium chelating agent
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Product Description

EGTA is a calcium chelator; protects against cell death caused by nitric oxide-induced calcium influx into nerve cells.

Product Specifications for EGTA

Molecular Weight

380.35

Formula

C14H24N2O10

Storage

Store at RT

Chemical Name

Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid

CAS Number

67-42-5

PubChem ID

6207

InChI Key

DEFVIWRASFVYLL-UHFFFAOYSA-N

SMILES

O=C(CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1M NaOH 38.03 100

Preparing Stock Solutions for EGTA

The following data is based on the product molecular weight 380.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.63 mL 13.15 mL 26.29 mL
5 mM 0.53 mL 2.63 mL 5.26 mL
10 mM 0.26 mL 1.31 mL 2.63 mL
50 mM 0.05 mL 0.26 mL 0.53 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 180 publications citing the usage of this product.

Product Documents for EGTA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EGTA

For research use only

Customer Reviews for EGTA (2)

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  • EGTA activates CRAC current via passive ER Ca2+ depletion in HEK293 cells co-expressing YFP-STIM1 and Orai1-CFP.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 12/16/2018
    ER Ca2+ in HEK293 cells co-expressing YFP-STIM1 and Orai1-CFP was passively depleted with pipette solution, containing (in mM): 20 EGTA, 145 Cs-methanesulfonate, 8 NaCl, 5 MgCl2 and 10 HEPES, pH 7.2 with KOH.
  • EGTA inhibits 15d-PGJ2 induced TNFa production by cardiomyocytes
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: cardiomyocytes
    Verified Customer | Posted 12/10/2018
    HL-1 cells were incubated with EGTA (5 mM) for 30 min prior to 15d-PGJ2 (15 µM) treatment for 4 h to follow TNFa concentrations in the cell culture medium. Addition of EGTA resulted in abolition of 15d-PGJ2 induced TNFa production.
    EGTA 2807

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