EIF2 beta Antibody

Novus Biologicals | Catalog # NB100-93284

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Primate (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all EIF2 beta Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 283 and 333 of human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa using the numbering given in entry NP_003899.2 (GeneID 8894).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF2 beta Antibody

Western Blot: EIF2 beta Antibody [NB100-93284]

Western Blot: EIF2 beta Antibody [NB100-93284]

Western Blot: EIF2 beta Antibody [NB100-93284] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-93284 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated less efficiently by NB100-93283, which recognizes an upstream epitope
Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284]

Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284]

Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2beta/EIF2S2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for EIF2 beta Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EIF2 beta

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]

Alternate Names

DKFZp686L18198, EIF2, EIF2B, EIF2beta, eIF-2-beta, eukaryotic initiation factor 2-beta, eukaryotic translation initiation factor 2 beta, eukaryotic translation initiation factor 2 subunit 2, Eukaryotic translation initiation factor 2 subunit beta, eukaryotic translation initiation factor 2, subunit 2 (beta, 38kD ), eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa, MGC8508

Entrez Gene IDs

8894 (Human); 67204 (Mouse)

Gene Symbol

EIF2S2

UniProt

P20042 (Human)

Additional EIF2 beta Products

Product Documents for EIF2 beta Antibody

Certificate of Analysis

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Product Specific Notices for EIF2 beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EIF2 beta Antibody

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Protocols

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