EndTag(TM) DNA/RNA End Labeling Kit
Novus Biologicals | Catalog # MB-9001-NB
![In-situ Hybridization: EndTag(TM) DNA/RNA End Labeling Kit [MB-9001-NB]](https://resources.rndsystems.com/images/products/EndTag-TM-DNA-RNA-End-Labeling-Kit-In-situ-Hybridization-MB-9001-NB-img0001.jpg "In-situ Hybridization: EndTag(TM) DNA/RNA End Labeling Kit [MB-9001-NB]")
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Key Product Details
Applications
Functional Assay
Kit Type
Labeling Kit
Product Summary for EndTag(TM) DNA/RNA End Labeling Kit
End labeling is a favored method for applications in which an internal label might interfere with hybridization or sequence specific protein binding.
Features:
Enables simple and uniform labeling of the 5' end of a DNA, RNA or unmodified oligonucleotide molecule.
Preserves the unmodified 3' end of the oligonucleotide (ideal for the labeling of PCR primers as a result).
Compatible with a choice of diverse labels - fluorophores, biotin, haptens, or affinity ligands*
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Product Specifications
Application Notes
Materials suitable to perform 10 end labeling reactions of up to 0.6 nmols of 5' ends (e.g. about 5 ug of a 25 base oligo) per reaction. *Note: Thiol-reactive labeling reagent is not included in the kit and must be purchased separately. Labels suitable for this kit include: Biotin (Long Arm) Maleimide, DNP Maleimide, Fluorescein (Long Arm) Maleimide, Fucose Maleimide, Texas Red(R) Maleimide..
Scientific Data Images for EndTag(TM) DNA/RNA End Labeling Kit
In-situ Hybridization: EndTag(TM) DNA/RNA End Labeling Kit [MB-9001-NB]
In-situ Hybridization: EndTag(TM) DNA/RNA End Labeling Kit [MB-9001-NB] - Fluorescence in situ hybridization of human chromosomes using 5' EndTag Fluorescein-labeled puC1.77 detected directly and mounted in VECTASHIELD Mounting Media with PI.
EndTag(TM) DNA/RNA End Labeling Kit [MB-9001-NB]
Kit Contents for EndTag(TM) DNA/RNA End Labeling Kit
- ATP gamma S
- Precipitant
- Reaction Buffer (10X)
- T4 polynucleotide kinase
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20 °C.
Product Documents for EndTag(TM) DNA/RNA End Labeling Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EndTag(TM) DNA/RNA End Labeling Kit
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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