Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # ENO/8632R

Format

Azide and BSA Free
View all Enolase 2/Neuron-specific Enolase Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human NSE gamma (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images

Enolase 2/Neuron-specific Enolase Antibody (ENO/8632R) - Azide and BSA Free

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO/8632R) - Azide and BSA Free [NBP3-49627] -

Western Blot Analysis of brain and heart lysates of different species using Enolase 2/Neuron-specific Enolase Antibody (ENO/8632R) - Azide and BSA Free.
Enolase 2/Neuron-specific Enolase Antibody (ENO/8632R) - Azide and BSA Free

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO/8632R) - Azide and BSA Free [NBP3-49627] -

Western Blot Analysis of human brain, human heart, A549, HeLa and Jurkat lysates of different species using Enolase 2/Neuron-specific Enolase Antibody (ENO/8632R) - Azide and BSA Free.

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Neuronal Markers

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Protocols

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FAQs

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