Enolase 2/Neuron-specific Enolase Antibody

Novus Biologicals | Catalog # NBP1-32090

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Enolase 2/Neuron-specific Enolase Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Enolase 2/Neuron-specific Enolase. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (83%), Chicken (89%).

Localization

Cytoplasm, Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody

Immunohistochemistry-Frozen: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090]

Immunohistochemistry-Frozen: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090]

Immunohistochemistry-Frozen: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] diluted at 1:250. Blue: Fluoroshield with DAPI.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090]

Western Blot: Enolase 1 Antibody [NBP1-32090] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090]

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-32090] - Paraffin-embedded PC14 xenograft, using antibody at 1:100 dilution.
Enolase 2/Neuron-specific Enolase Antibody

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] -

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 2/Neuron-specific Enolase antibody [N1C1] (NBP1-32090) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Enolase 2/Neuron-specific Enolase Antibody

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] -

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 2/Neuron-specific Enolase antibody [N1C1] (NBP1-32090) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Enolase 2/Neuron-specific Enolase Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers
Neuronal Markers

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Protocols

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