Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # KA1877
Key Product Details
Sample Type & Volume Required Per Well
Noradrenaline ELISA : 20 uL (extracted))
Sensitivity
Noradrenaline: 1.7 ng/mL Urine, 36 pg/mL Plasma
Assay Range
Product Specifications
Assay Type
Kit Type
Description
Precision
Intra-Assay Precision (Precision within an assay) Epinephrine: 12.57%, Norepinephrine: 12.95%
Inter-Assay Precision (Precision between assays) Epinephrine: 15.6%, Norepinephrine: 11.75%
Recovery for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
Recovery
Scientific Data Images for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
Kit Contents for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
- Acylation Buffer: Ready to use. Buffer with light alkaline pH for the acylation.
- Acylation Reagent: Ready to use. Acylation reagent in DMF and DMSO.
- Adhesive Foil: Ready to use. Adhesive foils in a resealable pouch.
- Adjustment Buffer: Ready to use, TRIS buffer.
- Adrenaline Antiserum: Ready to use, rabbit anti-adrenaline antibody, blue coloured.
- Adrenaline Microtiter Strips: Ready to use, antigen precoated microwell plate in a resealabel blue pouch with desiccant.
- Assay Buffer: Ready to use, 1 M hydrochloric acid and a non-mercury preservative.
- Coenzyme: Ready to use, S-adenosyl-L-methionine.
- Enzyme Conjugate: Ready to use, goat anti-rabbit immunoglobulins, conjugated with peroxidase.
- Enzyme: Lyophilized Catechol-O-methyltransferase.
- Extraction Buffer: Ready to use, buffer containing carbonate.
- Extraction Plate: Ready to use, coated with boronate affinity gel in a resealabel pouch.
- Hazards identification: H225 Highly flammable liquid and vapour. H360 May damage fertility or the unborn child. H319 Causes serious eye irritation
- Hydrochloric Acid: Ready to use, 0.025 M hydrochloric acid, yellow coloured.
- Noradrenaline Antiserum: Ready to use, rabbit anti-noradrenaline antibody, yellow coloured.
- Noradrenaline Microtiter Strips: Ready to use, antigen precoated microwell plate in a resealable yellow pouch with desiccant.
- Stop Solution: Ready to use, 0.25 M sulfuric acid. Hazards identification: H290 May be corrosive to metals. H314 Causes severe skin burns and eye damage.
- Substrate: Ready to use, chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide.
- Wash Buffer Concentrate: 50x concentrated buffer with a non-ionic detergent and physiological pH.
Preparation and Storage
Shipping
Stability & Storage
Background: Epinephrine/Norepinephrine
Alternate Names
Additional Epinephrine/Norepinephrine Products
Product Documents for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Citations for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
Powered by Bioz
Customer Reviews for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
There are currently no reviews for this product. Be the first to review Epinephrine/Norepinephrine ELISA Kit (Colorimetric) and earn rewards!
Have you used Epinephrine/Norepinephrine ELISA Kit (Colorimetric)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
-
Q: Can it be used with the samples from sheep?
A: The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.
-
Q: Can it be used with tissue homogenates?
A: This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.
-
Q: Can this kit be used with rat serum/plasma?
A: Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
-
Q: Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used
A: This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.
-
Q: Is this assay for use in rat plasma? The species is not listed.
A: The kit should work for any species as the target should be the same across all species.
-
Q: Can it be used with the samples from sheep?
A: The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.
-
Q: Can it be used with tissue homogenates?
A: This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.
-
Q: Can this kit be used with rat serum/plasma?
A: Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
-
Q: Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used
A: This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.
-
Q: Is this assay for use in rat plasma? The species is not listed.
A: The kit should work for any species as the target should be the same across all species.
-
Q: Can it be used with the samples from sheep?
A: The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.
-
Q: Can it be used with tissue homogenates?
A: This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.
-
Q: Can this kit be used with rat serum/plasma?
A: Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
-
Q: Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used
A: This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.
-
Q: Is this assay for use in rat plasma? The species is not listed.
A: The kit should work for any species as the target should be the same across all species.
-
Q: Can it be used with the samples from sheep?
A: The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.
-
Q: Can it be used with tissue homogenates?
A: This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.
-
Q: Can this kit be used with rat serum/plasma?
A: Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
-
Q: Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used
A: This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.
-
Q: Is this assay for use in rat plasma? The species is not listed.
A: The kit should work for any species as the target should be the same across all species.
-
Q: Can it be used with the samples from sheep?
A: The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.
-
Q: Can it be used with tissue homogenates?
A: This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.
-
Q: Can this kit be used with rat serum/plasma?
A: Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
-
Q: Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used
A: This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.
-
Q: Is this assay for use in rat plasma? The species is not listed.
A: The kit should work for any species as the target should be the same across all species.