![Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962]](https://resources.rndsystems.com/images/products/EPRS-Antibody-Immunohistochemistry-Paraffin-NBP3-12962-img0003.jpg "Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Drosophila
Predicted:
Bovine (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human EPRS. The exact sequence is proprietary.
Reactivity Notes
Mouse (86%), Rat (83%), Chicken (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
171 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for EPRS Antibody - BSA Free
Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962]
Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962] - EPRS antibody detects EPRS protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EPRS antibody (NBP3-12962) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962]](https://resources.rndsystems.com/images/products/EPRS-Antibody-Immunohistochemistry-Paraffin-NBP3-12962-img0002.jpg "Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962]")
Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962]
Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962] - EPRS antibody detects EPRS protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EPRS antibody (NBP3-12962) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunoprecipitation: EPRS Antibody [NBP3-12962]](https://resources.rndsystems.com/images/products/EPRS-Antibody-Immunoprecipitation-NBP3-12962-img0004.jpg "Immunoprecipitation: EPRS Antibody [NBP3-12962]")
Immunoprecipitation: EPRS Antibody [NBP3-12962]
Immunoprecipitation: EPRS Antibody [NBP3-12962] - Immunoprecipitation of EPRS protein from 293T whole cell extracts using 5 ug of EPRS antibody (NBP3-12962). Western blot analysis was performed using EPRS antibody (NBP3-12962) diluted at 1:2500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Western Blot: EPRS Antibody [NBP3-12962] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EPRS antibody (NBP3-12962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for EPRS Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EPRS
Alternate Names
bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, DKFZp313B047, EARS, EC 6.1.1, EC 6.1.1.15, EC 6.1.1.18, GLNS, GLUPRORS, glutamate tRNA ligase, glutaminyl-tRNA synthetase, glutamyl-prolyl-tRNA synthetase, PARSproliferation-inducing gene 32 protein, proliferation-inducing protein 32, proline tRNA ligase, proline-tRNA ligase, prolyl-tRNA synthetase, QARS, QPRS
Gene Symbol
EPRS1
Additional EPRS Products
Product Documents for EPRS Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EPRS Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for EPRS Antibody - BSA Free
There are currently no reviews for this product. Be the first to review EPRS Antibody - BSA Free and earn rewards!
Have you used EPRS Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...