ERManI Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP2-14216PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
View all ERManI Proteins and Enzymes »

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human MAN1B1.

Source: E. coli

Amino Acid Sequence: METGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKP

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14216.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP2-14216PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: ERManI

ERManI (MAN1B1) catalyzes the first mannose-trimming step in maturation of Asn-linked oligosaccharides. It trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2), specifically converting it to Man8GlcNAc isomer B. ERManI may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.

Alternate Names

alpha 1,2-mannosidase, EC 3.2.1.113, endoplasmic reticulum alpha-mannosidase 1, endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1, ER alpha 1,2-mannosidase, ER alpha-1,2-mannosidase, ER ManI, ER mannosidase 1, ERMan1, Man9GlcNAc2-specific processing alpha-mannosidase, Man9GlcNAc2-specific-processing alpha-mannosidase, MANA-ER, Mannosidase alpha class 1B member 1, mannosidase, alpha, class 1B, member 1, MRT15, RP11-350O14.2

Gene Symbol

MAN1B1

Additional ERManI Products

Product Documents for ERManI Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for ERManI Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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