ERP29 Antibody (1J0D3)

Novus Biologicals | Catalog # NBP3-15906

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1J0D3 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human ERP29 (P30040). MAAAVPRAAFLSPLLPLLLGFLLLSAPHGGSGLHTKGALPLDTVTFYKVIPKSKFVLVKFDTQYPYGEKQDEFKRLAENSASSDDLLVAEVGISDYGDKLNM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERP29 Antibody (1J0D3)

Western Blot: ERP29 Antibody (1J0D3) [NBP3-15906]

Western Blot: ERP29 Antibody (1J0D3) [NBP3-15906]

Western Blot: ERP29 Antibody (1J0D3) [NBP3-15906] - Western blot analysis of extracts of various cell lines, using ERP29 Rabbit mAb (NBP3-15906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
ERP29 Antibody (1J0D3)

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] -

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded mouse brain tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ERP29 Antibody (1J0D3)

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] -

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded human colon carcinoma tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ERP29 Antibody (1J0D3)

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] -

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded human liver tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ERP29 Antibody (1J0D3)

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] -

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded rat kidney tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ERP29 Antibody (1J0D3)

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] -

Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded human breast cancer tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for ERP29 Antibody (1J0D3)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERP29

ERP29 encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

C12orf8chromosome 12 open reading frame 8, endoplasmic reticulum lumenal protein ERp28, endoplasmic reticulum protein 29, Endoplasmic reticulum resident protein 28, endoplasmic reticulum resident protein 29, ERp28Endoplasmic reticulum resident protein 31, ERp29, ERp31ERP28, PDIA9, PDI-DB, protein disulfide isomerase family A, member 9

Gene Symbol

ERP29

Additional ERP29 Products

Product Documents for ERP29 Antibody (1J0D3)

Certificate of Analysis

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Product Specific Notices for ERP29 Antibody (1J0D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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