ERR alpha/NR3B1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16380

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (99%), Canine (98%), Mouse (98%), Porcine (99%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ERR alpha/NR3B1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERR alpha/NR3B1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERR alpha/NR3B1 Antibody - BSA Free

Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380]

Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380]

Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380] - Human intestine. ERR alpha stained by ERR alpha antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380]

Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380]

Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 ug of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary eagent.

Applications for ERR alpha/NR3B1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERR alpha/NR3B1

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]

Long Name

Estrogen-related Receptor alpha

Alternate Names

ERR1, ESRL1, ESRRA, NR3B1

Gene Symbol

ESRRA

UniProt

P11474 (Human)

Additional ERR alpha/NR3B1 Products

Product Documents for ERR alpha/NR3B1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ERR alpha/NR3B1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for ERR alpha/NR3B1 Antibody - BSA Free

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Protocols

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FAQs

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