EVI-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48848

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEY

Reactivity Notes

Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EVI-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EVI-1 Antibody [NBP2-48848]

Immunocytochemistry/ Immunofluorescence: EVI-1 Antibody [NBP2-48848]

Immunocytochemistry/Immunofluorescence: EVI-1 Antibody [NBP2-48848] - Staining of human cell line HEK 293 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848]

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
EVI-1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: EVI-1 Antibody - BSA Free [NBP2-48848]

Chromatin Immunoprecipitation-exo-Seq: EVI-1 Antibody - BSA Free [NBP2-48848]

ChIP-Exo-Seq composite graph for Anti-MECOM (NBP2-48848) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
EVI-1 Antibody - BSA Free

Western Blot: EVI-1 Antibody - BSA Free [NBP2-48848] -

Validation of MECOM expression levels in clinical KIRC samples. A Relative MECOM mRNA levels in tumor and adjacent normal tissues of 24 patients with KIRC. B MECOM mRNA differential expression in 24 patients with KIRC between tumor and adjacent normal tissues. C Representative images of MECOM IHC staining in tumor and adjacent normal tissues in patients with various T stages (n = 147). D IHC scores of MECOM expression in tumor tissues of patients with KIRC at various clinical stages. E MECOM protein levels in tumor and adjacent normal tissues of eight patients of KIRC Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39576394), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for EVI-1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EVI-1

EVI1 (ecotropic viral integration site 1) was originally identified as a gene located in the integration site of ecotropic retroviruses in the mouse genome that resulted in myeloid tumors. EVI1 is a zinc finger transcription factor that plays an important role in development and leukemogenesis. It is an oncogene that can have effects on cell proliferation, differentiation, and apoptosis. Chromosomal translocations involving EVI1 result in chronic myelogenous leukemia (CML). Alternate names for EVI1 include AML1-EVI-1 fusion protein, PRDM3, and MDS1-EV1.

Long Name

Ecotropic Viral Integration Site 1

Alternate Names

EVI1, MDS1, MECOM, PRDM3

Gene Symbol

MECOM

Additional EVI-1 Products

Product Documents for EVI-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EVI-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for EVI-1 Antibody - BSA Free

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Protocols

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