Exonuclease 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16391

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Exonuclease 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Exonuclease 1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Exonuclease 1 Antibody - BSA Free

Western Blot: Exonuclease 1 Antibody [NBP2-16391]

Western Blot: Exonuclease 1 Antibody [NBP2-16391]

Western Blot: Exonuclease 1 Antibody [NBP2-16391] - Sample (50 ug of whole cell lysate) A: mouse thymus, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391]

Immunocytochemistry/ Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391]

Immunocytochemistry/Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Exonuclease 1 Antibody - BSA Free

Western Blot: Exonuclease 1 Antibody [NBP2-16391] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody (NBP2-16391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Exonuclease 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391] -

Exonuclease 1 antibody detects Exonuclease 1 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Exonuclease 1 protein stained by Exonuclease 1 antibody (NBP2-16391) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Exonuclease 1 Antibody - BSA Free

Western Blot: Exonuclease 1 Antibody [NBP2-16391] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody (NBP2-16391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Exonuclease 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Exonuclease 1

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]

Alternate Names

Exo1, EXOI, Exonuclease I

Gene Symbol

EXO1

UniProt

Q9UQ84 (Human)

Additional Exonuclease 1 Products

Product Documents for Exonuclease 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Exonuclease 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Exonuclease 1 Antibody - BSA Free

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Protocols

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FAQs

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