FAM-DEALA-Hyp-YIPD

Tocris Bioscience | Catalog # 7287

Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Tocris Bioscience
Discontinued Product
7287 has been discontinued. View all Other Fluorescent Probes products.

Key Product Details

Description

Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity

Wavelength

Excitation = 485 nm, Emission = 520 nm
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Product Description

FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide (Kd = 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm. Longer peptide version, FAM-DEALAHypYIPMDDDFQLRSF (Cat. No. 7452), also available.

Optical Data

Emission Color Green
λabs 485 nm
λem 520 nm
Closest Laser line 488 nm
Application Fluorescence Polarization (FP) Displacement Assays

Spectra Viewer

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Product Specifications for FAM-DEALA-Hyp-YIPD

Molecular Weight

1477.48

Formula

C71H84N10O25

Storage

Store at -20°C

Purity

≥95% (HPLC)

InChI Key

CWNCTVVEODBMBK-RHNKRQRVSA-N

SMILES

O=C([C@@H]1CCCN1C([C@H]([C@@H](CC)C)NC([C@H](CC2=CC=C(O)C=C2)NC([C@@H]3C[C@@H](O)CN3C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C4=CC(C(O5)=O)=C(C=C4)C65C7=CC=C(O)C=C7OC8=C6C=CC(O)=C8)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O.O=C([C@@H]9CCCN9C([C@H]([C@@H](CC)C)NC([C@H](CC%10=CC=C(O)C=C%10)NC([C@@H]%11C[C@@H](O)CN%11C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C(C=C%12)=CC(C%13%14C%15=CC=C(O)C=C%15OC%16=C%13C=CC(O)=C%16)=C%12C(O%14)=O)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble to 1mg/ml in 0.01M PBS

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Product Documents for FAM-DEALA-Hyp-YIPD

Certificate of Analysis

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Product Specific Notices for FAM-DEALA-Hyp-YIPD

For research use only

Citations for FAM-DEALA-Hyp-YIPD

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