FAM-DEALAHypYIPMDDDFQLRSF

Tocris Bioscience | Catalog # 7452

Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Tocris Bioscience
Discontinued Product
7452 has been discontinued. View all Other Fluorescent Probes products.

Key Product Details

Description

Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity

Wavelength

Excitation = 485 nm, Emission = 520 nm
View all Other Fluorescent Probes »

Product Description

FAM-DEALAHypYIPMDDDFQLRSF is a 5-carboxyfluorescein (FAM) labeled HIF-1α peptide. It binds with high affinity to von Hippel-Lindau (VHL) protein (KD = 3 nM). Can be used to assess ligand binding to VHL in a direct fluorescence polarization (FP) displacement assay. Excitation/emission maxima (λ) = 485/520 nm. This product is a longer peptide version of FAM-DEALA-Hyp-YIPD (Cat. No. 7287).

Optical Data

Emission Color Green
λabs 485 nm
λem 520 nm
Closest Laser line 488 nm
Application Fluorescence Polarization (FP) Displacement Assays

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Product Specifications for FAM-DEALAHypYIPMDDDFQLRSF

Molecular Weight

2616.76

Formula

C122H158N24O39S

Storage

Store at -20°C

Purity

≥95% (HPLC)

InChI Key

BWRLQGDMCXAUCD-GMYGBYBYSA-N

SMILES

O=C(C1=CC(C(O2)=O)=C(C=C1)C32C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N6[C@@H](CC[C@H]6O)C(N[C@@H](CC7=CC=C(C=C7)O)C(N[C@@H]([C@@H](C)CC)C(N8[C@@H](CCC8)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC9=CC=CC=C9)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%10=CC=CC=C%10)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.O=C(C(C=C%11)=CC(C%12%13C%14=CC=C(O)C=C%14OC%15=C%12C=CC(O)=C%15)=C%11C(O%13)=O)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N%16[C@@H](CC[C@H]%16O)C(N[C@@H](CC%17=CC=C(C=C%17)O)C(N[C@@H]([C@@H](C)CC)C(N%18[C@@H](CCC%18)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC%19=CC=CC=C%19)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%20=CC=CC=C%20)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble to 1mg/ml in PBS (pH 7.4)

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Background References

References are publications that support the biological activity of the product.

Product Documents for FAM-DEALAHypYIPMDDDFQLRSF

Certificate of Analysis

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Product Specific Notices for FAM-DEALAHypYIPMDDDFQLRSF

For research use only

Citations for FAM-DEALAHypYIPMDDDFQLRSF

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