FARSB Antibody (OTI4B3)

Novus Biologicals | Catalog # NBP2-45577

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4B3
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Product Specifications

Immunogen

Full length human recombinant protein of human FARSB (NP_005678) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

65.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FARSB Antibody (OTI4B3)

Western Blot: FARSB Antibody (OTI4B3) [NBP2-45577]

Western Blot: FARSB Antibody (OTI4B3) [NBP2-45577]

Western Blot: FARSB Antibody (4B3) [NBP2-45577] - Analysis of extracts (10ug) from 5 different cell lines and 4 human tissue (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)
Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (4B3) [NBP2-45577] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (4B3) [NBP2-45577] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (4B3) [NBP2-45577] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (4B3) [NBP2-45577] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (4B3) [NBP2-45577] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (OTI4B3) [NBP2-45577]

Immunohistochemistry: FARSB Antibody (4B3) [NBP2-45577] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for FARSB Antibody (OTI4B3)

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FARSB

FARSB encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified.

Alternate Names

EC 6.1.1, EC 6.1.1.20, FARSLBphenylalanine-tRNA ligase beta chain, FRSBphenylalanine tRNA ligase 1, beta, cytoplasmic, PheHB, Phenylalanine--tRNA ligase beta chain, phenylalanine-tRNA synthetase-like, beta subunit, phenylalanyl-tRNA synthetase beta chain, phenylalanyl-tRNA synthetase beta-subunit, phenylalanyl-tRNA synthetase, beta subunit, phenylalanyl-tRNA synthetase-like, beta subunit, PheRS

Gene Symbol

FARSB

Additional FARSB Products

Product Documents for FARSB Antibody (OTI4B3)

Certificate of Analysis

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Product Specific Notices for FARSB Antibody (OTI4B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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