Fbx32 Antibody (1F10Z5)

Name: Fbx32 Antibody (1F10Z5)
Brand: Novus Biologicals
SKU: NBP3-16255
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16255

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1F10Z5 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Fbx32 (Q969P5). MPFLGQDWRSPGQNWVKTADGWKRFLDEKSGSFVSDLSSYCNKEVYNKENLFNSLNYDVAAKKRKKDMLNSKTKTQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSTAILDSRRFN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fbx32 Antibody (1F10Z5)

Western Blot: Fbx32 Antibody (1F10Z5) [NBP3-16255]

Western Blot: Fbx32 Antibody (1F10Z5) [NBP3-16255] - Western blot analysis of extracts of various cell lines, using Fbx32 Rabbit mAb (NBP3-16255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: Fbx32 Antibody (1F10Z5) [NBP3-16255]

Immunohistochemistry-Paraffin: Fbx32 Antibody (1F10Z5) [NBP3-16255] - Rat heart using Fbx32 Rabbit mAb (NBP3-16255) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Fbx32 Antibody (1F10Z5)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fbx32

Alternate Names

atrogin 1, ATROGIN1, atrogin-1, F-box only protein 32, F-box protein 32, Fbx32, FLJ32424, MAFBX, MAFbxMGC33610, Muscle atrophy F-box protein

Gene Symbol

FBXO32

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Product Documents for Fbx32 Antibody (1F10Z5)

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Certificate of Analysis

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Product Specific Notices for Fbx32 Antibody (1F10Z5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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