FerroOrange

Tocris Bioscience | Catalog # 8846

Sensitive and cell-permeable fluorogenic probe for the detection of labile Fe(II) in live cells; suitable for high-throughput assays
Tocris Bioscience

Key Product Details

Description

Sensitive and cell-permeable fluorogenic probe for the detection of labile Fe(II) in live cells; suitable for high-throughput assays

Alternative Names

RhoNox-4

Wavelength

Excitation = 545 nm, Emission = 575 nm
View all Ion Indicators »

Product Description

Key information:

FerroOrange (RhoNox-4) is a highly sensitive and robust fluorogenic probe for the detection of intracellular labile Fe(II). FerroOrange displays weak absorption and low fluorescence in the visible region, but becomes strongly fluorescent upon reaction with Fe(II).

Used for: detection of intracellular labile Fe(II).

Application: high-throughput assays for high content imaging of subcellular labile Fe(II) in live cells; fluorescent microscopy; studies on iron homeostasis and ferroptosis.

Properties and Photophysical Data: FerroOrange has excellent cell-membrane permeability and low cell toxicity. FerroOrange has no chelating ability. For the fluorescent form, excitation and emission maxima (λ) measured in 50 mM HEPES buffer (pH 7.4, 0.2% DMSO) are 545 nm and 575 nm, respectively; extinction coefficient ε = 73,500 M-1cm-1; quantum yield φ = 0.19.

Please refer to this protocol for further information on how to use this product.

Optical Data

Emission Color Orange
λabs 545 nm
λem 575 nm
Extinction Coefficient (ε) 73500 M-1cm-1
Quantum Yield (φ) 0.19
Cell Permeable Yes
Application High-throughput assays for the detection of intracellular labile Fe(II)

Product Specifications for FerroOrange

Molecular Weight

684.78

Formula

C38H44N4O8

Storage

Store at +4°C

Purity

≥90% (HPLC)

Chemical Name

4-(tert-Butoxycarbonyl)-1-(3'-(4-(tert-butoxycarbonyl)piperazin-1-yl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6'-yl)piperazine 1-oxide

CAS Number

2711762-70-6

PubChem ID

172913725

InChI Key

BPPNJBCDCLJSAA-UHFFFAOYSA-N

SMILES

O=C1OC2(C3=C1C=CC=C3)C4=CC=C(N5CCN(CC5)C(OC(C)(C)C)=O)C=C4OC6=CC([N+]7(CCN(CC7)C(OC(C)(C)C)=O)[O-])=CC=C62

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for FerroOrange

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FerroOrange

For research use only

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