Recombinant Human FGF-4 Protein

Novus Biologicals | Catalog # NBP2-34864

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P08620

Applications

Bioactivity, SDS-PAGE
View all FGF-4 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 182 amino acids corresponding to FGF-4 Source: E. coli

Uniprot ID: P08620

Amino Acid Sequence: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-4 as determined by LAL method.

Predicted Molecular Mass

19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fibroblast Growth Factor 4 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBP2-34864 in the following applications:

Scientific Data Images for Recombinant Human FGF-4 Protein

SDS-PAGE: Recombinant Human FGF-4 Protein [NBP2-34864]

SDS-PAGE: Recombinant Human FGF-4 Protein [NBP2-34864]

SDS-Page: Human FGF-4 Protein [NBP2-34864]

Formulation, Preparation, and Storage

NBP2-34864
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, 350 mM NaCl, 0.02% Tween-20, pH 7.0.
Preservative 5% Trehalose
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-4

FGF4, also named FGFK and KFGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF4 shares about 30 % sequence identity with the prototypical members of the FGF family.)

Long Name

Fibroblast Growth Factor 4

Alternate Names

FGF4, HBGF-4, HST-1, KFGF, KS3

Gene Symbol

FGF4

UniProt

P08620

Additional FGF-4 Products

Product Documents for Recombinant Human FGF-4 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human FGF-4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human FGF-4 Protein

Customer Reviews for Recombinant Human FGF-4 Protein (1)

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  • Human FGF-4 Protein
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 01/04/2017
    SDS PAGE gel with Coomassie blue staining showing detection of Human FGF-4 Protein (NBP2-34864-25 ug) (3 ug).
    Recombinant Human FGF-4 Protein NBP2-34864

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