Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-43555

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 59D8

Format

BSA Free
View all Fibrin beta-chain Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by using a synthetic heptapeptide from the amino terminus of the beta chain in human fibrin as an immunogen. (Uniprot# P02675)

Specificity

This antibody selectively binds to the amino-terminus of the fibrin beta-chain that becomes exposed after cleavage of fibrinopeptide B by thrombin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Scientific Data Images for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free

Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free

Immunocytochemistry /Immunofluorescence: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] -

Immunocytochemistry /Immunofluorescence: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix coverslips stained with NBP3-43555 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43555, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free

Western Blot: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] -

Western Blot: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] - Human liver (A), placenta (B), and serum (C) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43555 at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Applications for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibrin beta-chain

Alternate Names

Beta-Fibrinogen, epididymis secretory sperm binding protein Li 78p, fibrinogen beta chain, fibrinogen, B beta polypeptide, HEL-S-78p

Gene Symbol

FGB

Additional Fibrin beta-chain Products

Product Documents for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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