Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81111

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, In vivo assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 8E5

Format

Azide and BSA Free
View all Fibrin beta-chain Primary Antibodies »

Product Specifications

Immunogen

8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).

Specificity

8E5 binds to the amino-terminal region of the beta-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. This peptide sequence is found only in fibrin, and not in its precursor fibrinogen and so there is no cross-reactivity with fibrinogen. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free

Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free [NBP2-81111]

Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free [NBP2-81111]

Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric [NBP2-81111] - Flow-cytometry using the Fibrin beta-chain Antibody [NBP2-81111]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 8E5 (NBP2-81111, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Fibrin beta-chain

Alternate Names

Beta-Fibrinogen, epididymis secretory sperm binding protein Li 78p, fibrinogen beta chain, fibrinogen, B beta polypeptide, HEL-S-78p

Gene Symbol

FGB

Additional Fibrin beta-chain Products

Product Documents for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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