Human Fibronectin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-83737

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, heparin), urine (50 uL)

Sensitivity

0.1 ng/mL

Assay Range

0.31 - 20.0 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into positive serum. There was no crossreactivity detected.

Description

The human Fibronectin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Fibronectin

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.3%

Inter-Assay Precision (Precision between assays) CV% 6.7%

Recovery for Human Fibronectin ELISA Kit (Colorimetric)

Recovery

110.6%

Scientific Data Images for Human Fibronectin ELISA Kit (Colorimetric)

ELISA: Human Fibronectin ELISA Kit (Colorimetric) [NBP1-83737]

ELISA: Human Fibronectin ELISA Kit (Colorimetric) [NBP1-83737]

ELISA: Fibronectin/Anastellin ELISA Kit [NBP1-83737] - Standard curve

Kit Contents for Human Fibronectin ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Fibronectin
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human Fibronectin polyclonal antibody
  • Human Fibronectin Standard lyophilized, 40 ng/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Fibronectin

Fibronectin (also known as FN1, or Fibronectin 1) is an extracellular matrix glycoprotein that binds to integrins and has a large role in cell adhesion, migration, differentiation, and growth. It plays a key role in wound healing by helping form blood clots and participates in embryonic development by guiding cell attachment and migration. Fibronectin consists of two monomers linked by disulfide bonds and binds extracellular components such as collagen fibrin and syndecans. Fibronectin has been implicated in carcinoma and tumor development while also limiting tumor cells response to treatment. Novus offers Fibronectin antibodies, ELISA Kits, lysates, and proteins

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Human Fibronectin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Fibronectin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human Fibronectin ELISA Kit (Colorimetric)

Showing  1 - 2 of 2 FAQs Showing All

    • Q: I would like to know what was used for the Fibronectin Standard. A recombinant protein, or a human native protein?
      A: NBP1-83737 contains a human fibronectin protein standard, which was purified from plasma.

    • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
      A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 
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