Filipin III

Tocris Bioscience | Catalog # 6250

Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy
Tocris Bioscience

Key Product Details

Description

Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy
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Product Description

Key information: filipin III is a fluorescent cholesterol stain; polyene antibiotic.

Used for: fluorescent cholesterol staining. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form.

Application: confocal microscopy.

Properties and Photophysical Data: excitation and emission maxima (λ) are 405 nm and 420-480 nm, respectively.

Product Specifications for Filipin III

Molecular Weight

654.83

Formula

C35H58O11

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

(3R,4S,6S,8S,10R,12R,14R,16S,17E,19E,21E,23E,25E,27S,28R)-4,6,8,10,12,14,16,27-Octahydroxy-3-[(1R)-1-hydroxyhexyl]-17,28-dimethyloxacyclooctacosa-17,19,21,23,25-pentaen-2-one

CAS Number

480-49-9

PubChem ID

6433553

InChI Key

IMQSIXYSKPIGPD-ZPXVTHDFSA-N

SMILES

C[C@@H](O1)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@]([C@H](O)CCCCC)([H])C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.27 5

Preparing Stock Solutions for Filipin III

The following data is based on the product molecular weight 654.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 30.54 mL 152.71 mL 305.42 mL
0.25 mM 6.11 mL 30.54 mL 61.08 mL
0.5 mM 3.05 mL 15.27 mL 30.54 mL
2.5 mM 0.61 mL 3.05 mL 6.11 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for Filipin III

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Filipin III

For research use only

Customer Reviews for Filipin III (1)

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  • Staining for intracellular cholesterol
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: MEF
    Verified Customer | Posted 06/13/2019
    Fix the cells with 4% PFA for 15 min at room temperature; permeabilisation not required; incubate the cells with 0.5 mg/mL Fillipin in 3% BSA for 4 h at room temperature; mount the coverslips with DAPI-free moviol. Excite the cells with 405 nm laser, and collect signals at 410-500 nm.
    Filipin III 6250

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