FITC

Tocris Bioscience | Catalog # 5440

Green fluorescent dye
Tocris Bioscience

Key Product Details

Description

Green fluorescent dye

Alternative Names

Fluorescein-5-Isothiocyanate,FITC Isomer 1

Wavelength

Excitation = 495 nm, Emission = 525 nm
View all Standard Fluorescein, Coumarin and Rhodamine-based »

Product Description

Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines. FITC is bright and has good water-solubility, however it is sensitive to changes in pH and temperature, and has a relatively high photobleaching rate.

Application: Suitable for flow cytometry, immunofluorescence (IHC, ICC), fluorescence microscopy, fluorescence in situ hybridization (FISH), biochemical assays.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 495 nm and 525 nm, respectively; quantum yield = 0.92; extinction coefficient = 75,000 M-1cm-1; A280 = 0.35.

Optical Data

FITC Dye Spectra
Emission Color Green
Brightness
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group Isothiocyanate
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.35
Application Flow Cytometry, Immunofluorescence (IHC, ICC), Fluorescence Microscopy, FISH

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for FITC

Molecular Weight

389.38

Formula

C21H11NO5S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

CAS Number

3326-32-7

PubChem ID

18730

InChI Key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

SMILES

OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.94 100

Preparing Stock Solutions for FITC

The following data is based on the product molecular weight 389.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.84 mL 25.68 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.28 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 16 publications citing the usage of this product.

Product Documents for FITC

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FITC

For research use only

Customer Reviews for FITC

There are currently no reviews for this product. Be the first to review FITC and earn rewards!

Have you used FITC?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card