FITC

Catalog # Availability Size / Price Qty
5440/50
FITC | CAS No. 3326-32-7 | Standard Fluorescein, Coumarin and Rhodamine-based
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Description: Green fluorescent dye
Alternative Names: Fluorescein-5-Isothiocyanate, FITC Isomer 1

Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

Purity: ≥98%

Product Details
Citations (1)
Reviews

Biological Activity

Fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm.

Optical Data for FITC

FITC Dye Spectra
Emission Color Green
Brightness
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group Isothiocyanate
Reactivity Primary amines
Cell Permeable Yes

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
389.38
Formula:
C21H11NO5S
Solubility:
Soluble to 100 mM in DMSO and to 20 mM in ethanol
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
3326-32-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Citation for FITC

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