Recombinant Flagellin protein FliA(H) Protein

Novus Biologicals | Catalog # NBP2-61446

Novus Biologicals
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Product Specifications

Description

A single non-glycosylated polypeptide chain containing 302 amino acids corresponding to Flagellin protein FliA(H) Source: E. coli

Uniprot ID: Q8RR94

Amino Acid Sequence: MKGLKTGWIE KSVENIKTAY GIEPTGANKL KVTISDDGAY GVLASVTPKT GEFELHIDSS DFEKGDGESG NNIHGKLYDD RIIQHEMTHA VMNDALGIDK MNDLHDKNKL WFIEGTAEAM AGADERVKDI IGNDTQTGID NTKLSKLATR ADALLNGVSW NSSDEDYAAG YLMVKYIASK GIDLKAVMKE IKNTGASGLD NKIDLTNLKI DFKNNLENYI KDISKVHLDW DDDEKDVGSI LGSDHGHGDI KAEDVVKGTT PEKEQPLDKF KIIWPDDNSD NTTGKIQLQV GANEGQSITI LE

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Flagellin protein FliA(H) as determined by LAL method.

Predicted Molecular Mass

33.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-61446
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Flagellin protein FliA(H)

Flagellin protein FliA(H), also named RNA polymerase sigma factor for flagellar operon, Sigma F and Sigma-28, is belonging to the sigma-70 factor family or FliA subfamily. Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor controls the expression of flagella-related genes. May regulate the expression of genes involved in virulence.

Alternate Names

FliA, RNA polymerase sigma factor FliA, Sigma F, Sigma-27, Sigma-28

Gene Symbol

fliA(H)

Additional Flagellin protein FliA(H) Products

Product Documents for Recombinant Flagellin protein FliA(H) Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Flagellin protein FliA(H) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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