Fluo-4 AM

Catalog # Availability Size / Price Qty
Fluo-4 AM | CAS No. 273221-67-3 | Calcium Signaling Probes and Ca2+ Indicator Dyes
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Description: Cell-permeable, fluorescent Ca2+ indicator

Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester

Purity: ≥95%

Product Details
Reviews (2)

Biological Activity

Cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.

Technical Data

Soluble to 1 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes.
    Gee et al.
    Cell Calcium., 2000;27:97

Product Datasheets

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Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells.
By Niroj Shrestha on 12/24/2019
Application: Species: Rat

RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.

works well in motor neurons
By Karthik krishnamurthy on 10/24/2018
Application: Species: Rat

Used for imaging calcium entry in motor neurons at 1 mM concentration

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