Fluo-4 AM New
Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
Biological ActivityCell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes.
Gee et al.
Cell Calcium., 2000;27:97
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Used for imaging calcium entry in motor neurons at 1 mM concentration