Fluo-4 AM

Tocris Bioscience | Catalog # 6255

Cell-permeable, fluorescent Ca2+ indicator
Tocris Bioscience

Key Product Details

Description

Cell-permeable, fluorescent Ca2+ indicator

Wavelength

Excitation = 494 nm, Emission = 506 nm
View all Calcium Signaling Probes and Ca2+ Indicator Dyes »

Product Description

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.

It is recommended to prepare stock solutions in DMSO.

Optical Data

Emission Color Green
λabs 494 nm
λem 506 nm
Closest Laser line 488 nm
Cell Permeable Yes
Application Fluorescence Microscopy, Flow Cytometry, Fluorometric Microplate Reading Assays

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Product Specifications for Fluo-4 AM

Molecular Weight

1096.95

Formula

C51H50F2N2O23

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester

CAS Number

273221-67-3

PubChem ID

4060965

InChI Key

QOMNQGZXFYNBNG-UHFFFAOYSA-N

SMILES

O=C(C(F)=C1)C=C2C1=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)C5=CC(F)=C(OCOC(C)=O)C=C5O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 23 publications citing the usage of this product.

Product Documents for Fluo-4 AM

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluo-4 AM

For research use only

Customer Reviews for Fluo-4 AM (2)

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  • Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells.
    Name: Niroj Shrestha
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: RBL-2H3
    Verified Customer | Posted 12/24/2019
    RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
    Fluo-4 AM 6255
  • works well in motor neurons
    Name: Karthik krishnamurthy
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: motor neurons
    Verified Customer | Posted 10/24/2018
    motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.
    Used for imaging calcium entry in motor neurons at 1 mM concentration
    Fluo-4 AM 6255

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