Chemical Name: 3',6'-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(6)-carboxamide
Biological ActivityFluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
Optical Data for Fluorescein Tyramide
|Extinction Coefficient (ε)||70000 M-1cm-1|
|Closest Laser line||488 nm|
|Application||Tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH|
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.
Bobrow et al.
Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry.
Karkmann et al.
Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size.
Schriml et al.
Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification.
Hopman et al.
Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization.
van Gijlswijk et al.
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