FSH beta Antibody (SPM107) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-47735

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM107

Format

Azide and BSA Free
View all FSH beta Primary Antibodies »

Product Specifications

Immunogen

Full length Purified Human FSH beta (Uniprot: P01225)

Localization

Cytoplasmic, secreted

Specificity

This monoclonal antibody reacts with a protein of 22kDa, identified as beta sub-unit of FSH. It does not cross react with the alpha sub-unit. Follicle stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotrophs in the anterior pituitary gland. In the ovary, FSH stimulates the growth of immature Graafian follicles to maturation. As the follicle grows, it releases inhibin, which deactivates the FSH production. In men, FSH enhances the production of androgen-binding protein by the Sertoli cells of the testis and is critical for spermatogenesis. FSH and LH act synergistically in reproduction. FSH is a useful marker in the classification of pituitary tumors and the study of pituitary disease.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45133).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for FSH beta Antibody (SPM107) - Azide and BSA Free

Immunohistochemistry-Paraffin: FSH beta Antibody (SPM107) - Azide and BSA Free [NBP2-47735]

Immunohistochemistry-Paraffin: FSH beta Antibody (SPM107) - Azide and BSA Free [NBP2-47735]

Immunohistochemistry-Paraffin: FSH beta Antibody (SPM107) - Azide and BSA Free [NBP2-47735] - Human Pituitary stained with FSH-beta Monoclonal Antibody (SPM107).

Applications for FSH beta Antibody (SPM107) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: FSH beta

FSH is a pituitary hormone involved in the maturation of ovarian follicles and estrogen secretion in females. In the pituitary gland, FSH is produced by gonadotrophs. In males, FSH stimulates the secretion of testosterone. Follicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one (see also luteinizing hormone, chorionic gonadotropin, and thyroid stimulating hormone), consist of a shared alpha chain and a beta chain encoded by a separate gene.

Long Name

Follicle Stimulating Hormone, beta

Alternate Names

Follitropin, FSHB

Gene Symbol

FSHB

Additional FSH beta Products

Product Documents for FSH beta Antibody (SPM107) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FSH beta Antibody (SPM107) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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