FXYD5/Dysadherin Antibody (M029)

Name: FXYD5/Dysadherin Antibody (M029)
Brand: Novus Biologicals
SKU: NBP3-23349
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23349

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # M029

View all FXYD5/Dysadherin Primary Antibodies »

Product Specifications

Immunogen

Clone (M029) was generated from a proprietary antigen related to the native human FXYD5/Dysadherin protein expressed in A431 epidermoid carcinoma cell line. [UniProt# Q96DB9]

Specificity

Clone M029 mouse monoclonal antibody detects a 35-55 kDa band on SDS-PAGE immunoblots of human A431, A549, MeWo, MDA-MB-231 cell, as well as human breast, lung, and skin tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for FXYD5/Dysadherin Antibody (M029)

Western Blot: Mouse FXYD5/Dysadherin mAb (M029) [NBP3-23349]

Western Blot: Mouse FXYD5/Dysadherin mAb (M029) [NBP3-23349] - Analysis of FXYD5/Dysadherin protein expression. Immunoprecipitates using FXYD5/Dysadherin Antibody (M031) (Catalog # NBP3-23350) only (lane 1) or FXYD5/Dysadherin Antibody (M031) with A431 lysate (lane 2) or A431 input only (lane 3). Human cell lysates MDA-MB-231 (lane 4) or MeWo (lane 5). The blot was probed with Mouse FXYD5/Dysadherin mAb (M029) at 1:1000.

Immunocytochemistry/Immunofluorescence: Mouse FXYD5/Dysadherin mAb (M029) [NBP3-23349]

Immunocytochemistry/Immunofluorescence: Mouse FXYD5/Dysadherin mAb (M029) [NBP3-23349] - Labeling of FXYD5/Dysadherin in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with Mouse FXYD5/Dysadherin mAb (M029). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Applications for FXYD5/Dysadherin Antibody (M029)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: FXYD5/Dysadherin

Dysadherin encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane.

Long Name

FXYD Domain-containing Ion Transport Regulator 5

Alternate Names

DYSAD, Dysadherin, EF-8, IWU1, KCT1, OIT2, PRO6241, RIC

Gene Symbol

FXYD5

Additional FXYD5/Dysadherin Products

Product Documents for FXYD5/Dysadherin Antibody (M029)

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Certificate of Analysis

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Product Specific Notices for FXYD5/Dysadherin Antibody (M029)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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