Biological ActivityAminoglycosidic antibiotic. Inhibits protein synthesis in both prokaryotic and eukaryotic cells. Used for selection of eukaryotic cells transfected with the neo gene.
This product has been cell culture tested.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
- This product has been cell culture tested
Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation and preliminary characterization.
Wagman et al.
Antimicrob.Agents Chemother., 1974;6:144
G-418, an elongation inhibitor of 80 S ribosomes.
Bar-Nun et al.
Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromo.
Burke and Mogg
Nucleic Acids Res., 1985;13:6265
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G418 was reconstituted in milli-Q water to get a stock solution of 50 mg/ml. The stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to culture HEK293 cell line, stably expressing hRyR2-IRES-GFP.