G418 disulfate salt

Tocris Bioscience | Catalog # 4131

Aminoglycoside antibiotic; used in cell culture
Tocris Bioscience
Discontinued Product
4131 has been discontinued. View all Antibiotics products.

Key Product Details

Description

Aminoglycoside antibiotic; used in cell culture
View all Antibiotics »

Product Description

G418 disulfate salt is an aminoglycosidic antibiotic. Inhibits protein synthesis in both prokaryotic and eukaryotic cells. Used for selection of eukaryotic cells transfected with the neo gene.

This product has been cell culture tested.

Product Specifications for G418 disulfate salt

Molecular Weight

692.71

Formula

C20H40N4O10.2H2SO4

Storage

Store at +4°C

CAS Number

108321-42-2

PubChem ID

206347

InChI Key

UHEPSJJJMTWUCP-DHDYTCSHSA-N

SMILES

N[C@H]1[C@H](O[C@]2([H])[C@H](O)[C@@H](NC)[C@@](C)(O)CO2)[C@@H](O)[C@H](O[C@]3([H])[C@H](N)[C@@H](O)[C@H](O)[C@]([C@H](O)C)([H])O3)[C@@H](N)C1.OS(O)(=O)=O.OS(O)(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 51.95 75

Preparing Stock Solutions for G418 disulfate salt

The following data is based on the product molecular weight 692.71.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 1.92 mL 9.62 mL 19.25 mL
3.75 mM 0.38 mL 1.92 mL 3.85 mL
7.5 mM 0.19 mL 0.96 mL 1.92 mL
37.5 mM 0.04 mL 0.19 mL 0.38 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 7 publications citing the usage of this product.

Product Documents for G418 disulfate salt

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for G418 disulfate salt

For research use only

Citations for G418 disulfate salt

Customer Reviews for G418 disulfate salt (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used G418 disulfate salt?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • G418 maintains stable expression of recombinant hRyR2 in HEK293 cells.
    Name: Niroj Shrestha
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 01/26/2019
    Stock solutions should be stored in aliquots at -20°C.
    G418 was reconstituted in milli-Q water to get a stock solution of 50 mg/ml. The stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to culture HEK293 cell line, stably expressing hRyR2-IRES-GFP.
    G418 disulfate salt 4131

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All