G9a/EHMT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55881

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G9a/EHMT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PRSEETLPKATPDSLEPAGPSSPASVTVTVGDEGADTPVGATPLIGDESENLEGDGDLRGGRILLGHATKSFPSS

Reactivity Notes

Mouse 85%, Rat 87%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G9a/EHMT2 Antibody - BSA Free

Western Blot: G9a/EHMT2 Antibody [NBP2-55881]

Western Blot: G9a/EHMT2 Antibody [NBP2-55881]

Western Blot: G9a/EHMT2 Antibody [NBP2-55881] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881]

Immunocytochemistry/ Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881]

Immunocytochemistry/Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear speckles. Antibody staining is shown in green.
G9a/EHMT2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: G9a/EHMT2 Antibody - BSA Free [NBP2-55881]

Chromatin Immunoprecipitation-exo-Seq: G9a/EHMT2 Antibody - BSA Free [NBP2-55881]

ChIP-Exo-Seq composite graph for Anti-EHMT2 (NBP2-55881) tested in HEK293 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for G9a/EHMT2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G9a/EHMT2

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Long Name

G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2

Alternate Names

EHMT2

Gene Symbol

EHMT2

Additional G9a/EHMT2 Products

Product Documents for G9a/EHMT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for G9a/EHMT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Notch Pathway

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail