GCH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84949

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GCH1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCH1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GCH1 Antibody [NBP1-84949]

Immunocytochemistry/ Immunofluorescence: GCH1 Antibody [NBP1-84949]

Immunocytochemistry/Immunofluorescence: GCH1 Antibody [NBP1-84949] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
GCH1 Antibody

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] -

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
GCH1 Antibody

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] -

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
GCH1 Antibody

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] -

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] -Staining of human pancreas shows strong cytoplasmic and nuclear positivity in islets of Langerhans.
GCH1 Antibody

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] -

Immunohistochemistry-Paraffin: GCH1 Antibody [NBP1-84949] -Staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.

Applications for GCH1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCH1

GCH1 encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme.

Long Name

GTP Cyclohydrolase 1

Alternate Names

DYT5, DYT5a, GTP-CH-1, GTP-CH-I, GTPCH1

Entrez Gene IDs

2643 (Human)

Gene Symbol

GCH1

UniProt

P30793 (Human)

Additional GCH1 Products

Product Documents for GCH1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GCH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GCH1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GCH1 Antibody - BSA Free

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  • Q: Do you sell this product at larger amount in one package at a lower price? Thanks. For example 200ul at $500

    A:

    In regards to your inquiry about pricing for larger amounts unfortunately we do not provide that. We can provide bulk discounts for the purchase of multiple vials, but typically that starts at 10 or more vials. If you wish to contact bulk@novusbio.com they can provide a quote for large purchases. We also have promotions that you might find useful.

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