GEMIN2 Antibody (OTI4C11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71374

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C11

Format

Azide and BSA Free
View all GEMIN2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GEMIN2(NP_003607) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GEMIN2 Antibody (OTI4C11) - Azide and BSA Free

Western Blot: GEMIN2 Antibody (OTI4C11)Azide and BSA Free [NBP2-71374]

Western Blot: GEMIN2 Antibody (OTI4C11)Azide and BSA Free [NBP2-71374]

Western Blot: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GEMIN2.
Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374]

Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374]

Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374]

Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374]

Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374]

Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374]

Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Applications for GEMIN2 Antibody (OTI4C11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GEMIN2

SIP1 encodes one of the proteins found in the SMN complex, which consists of the suvival of motor neuron protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

Alternate Names

Component of gems 2, gemin-2, GEMIN2survival of motor neuron protein-interacting protein 1, SIP1-delta, SMN interacting protein 1-delta, SMN-interacting protein 1, survival of motor neuron protein interacting protein 1

Gene Symbol

GEMIN2

Additional GEMIN2 Products

Product Documents for GEMIN2 Antibody (OTI4C11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GEMIN2 Antibody (OTI4C11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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