GF-AFC

Tocris Bioscience | Catalog # 8143

Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays
Tocris Bioscience

Key Product Details

Description

Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays
View all Enzyme Probes and Enzyme Substrates »

Product Description

GF-AFC (glycyl-phenylalanyl-aminofluorocoumarin) is a cell permeable peptide fluorogenic substrate. GF-AFC is cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays. GF-AFC can be used as a substrate for measuring the activity of dipeptidyl peptidase 1 (DPP1), an intracellular lysosomal cysteinyl protease.

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Product Specifications for GF-AFC

Molecular Weight

469.85

Formula

C21H18F3N3O4.HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(2S)-2-[(2-Aminoacetyl)amino]-N-[2-oxo-4-(trifluoromethyl)chromen-7-yl]-3-phenylpropanamide hydrochloride

InChI Key

GBCCOIYISILSJP-NTISSMGPSA-N

SMILES

NCC(N[C@@H](CC1=CC=CC=C1)C(NC2=CC=C(C(O3)=C2)C(C(F)(F)F)=CC3=O)=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 46.98 100

Preparing Stock Solutions for GF-AFC

The following data is based on the product molecular weight 469.85.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.13 mL 10.64 mL 21.28 mL
5 mM 0.43 mL 2.13 mL 4.26 mL
10 mM 0.21 mL 1.06 mL 2.13 mL
50 mM 0.04 mL 0.21 mL 0.43 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for GF-AFC

Certificate of Analysis

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Product Specific Notices for GF-AFC

For research use only

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