Glutaminase Activity Assay Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-24496

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, animal tissue and plant tissue

Sensitivity

0.003 U/L

Assay Range

0.003 - 18.0 U/L
View all Glutaminase Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Species

Non-species specific

Description

Detection instrument: Microplate reader (440 - 460 nm, optimum wavelength: 450 nm)
Assay time: 80 min

Precision

Intra-Assay Precision (Precision within an assay) %CV 5

Inter-Assay Precision (Precision between assays) %CV 8.3

Scientific Data Images for Glutaminase Activity Assay Kit (Colorimetric)

Glutaminase Activity Assay Kit (Colorimetric)

Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] -

Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] - Standard Reference Curve
Glutaminase Activity Assay Kit (Colorimetric)

Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] -

Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] - Assay Example

Kit Contents for Glutaminase Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Substrate A
  • Reagent 2: Standard
  • Reagent 3: Diluent
  • Reagent 4: Enzyme Reagent
  • Reagent 5: Buffer Solution
  • Reagent 6: Substrate B
  • Reagent 7: Accelerator
  • Reagent 8: Chromogenic Agent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutaminase

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Gene Symbol

GLS

Additional Glutaminase Products

Product Documents for Glutaminase Activity Assay Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Glutaminase Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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