Granzyme M Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP2-14079PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
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Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human GZMM.

Source: E. coli

Amino Acid Sequence: PSMVCLAADSKDQAPCKGDSGGPLVCGKGRVLAGVLSFSSRVCTDIFKPPVATAVAPYVSWIRKVTGR

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14079.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP2-14079PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: Granzyme M

The GZMM gene encodes a 257 amino acid long, 27 kDA granzyme M protein that cleaves peptide substrates after methionine, leucine, and norleucine. In doing so, it encourages caspase activation and apoptosis of target cells. GZMM has been investigated for its role in arrhythmogenic right ventricular dysplasia as it participates in the immune system, alternative complement activation, and granzyme pathways. It is known to interact with genes CASP3, DFFA, EZR, NPM1, and PAK2.

Alternate Names

EC 3.4.21, EC 3.4.21.-, granzyme M, granzyme M (lymphocyte met-ase 1), HU-Met-1, LMET1, lymphocyte met-ase 1, Met-1 serine protease, MET1met-ase, Met-ase, Natural killer cell granular protease

Gene Symbol

GZMM

Additional Granzyme M Products

Product Documents for Granzyme M Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for Granzyme M Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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