GRASP65 Antibody (3F2I1)

Novus Biologicals | Catalog # NBP3-16113

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3F2I1 expressed in HEK293
View all GRASP65 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 341-440 of human GRASP65 (Q9BQQ3). SGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRASP65 Antibody (3F2I1)

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
GRASP65 Antibody (3F2I1)

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] -

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Confocal imaging of U-2 OS cells using GRASP65 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
GRASP65 Antibody (3F2I1)

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] -

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Confocal imaging of Hep G2 cells using GRASP65 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for GRASP65 Antibody (3F2I1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRASP65

GRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis.

Alternate Names

Golgi peripheral membrane protein p65, Golgi phosphoprotein 5MGC118897, golgi reassembly stacking protein 1, 65kDa, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GRASP65MGC118894, P6565 kDa

Gene Symbol

GORASP1

Additional GRASP65 Products

Product Documents for GRASP65 Antibody (3F2I1)

Certificate of Analysis

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Product Specific Notices for GRASP65 Antibody (3F2I1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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