Human GRB 14 ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) CV% 10

Inter-Assay Precision (Precision between assays) CV% 10

Recovery for Human GRB 14 ELISA Kit (Colorimetric)

Linearity

Scientific Data Images for Human GRB 14 ELISA Kit (Colorimetric)

Kit Contents for Human GRB 14 ELISA Kit (Colorimetric)

• Biotinylated Detection Ab Diluent
• Certificate of Analysis
• Concentrated Biotinylated Detection Ab (100x)
• Concentrated HRP Conjugate (100x)
• Concentrated Wash Buffer (25x)
• HRP Conjugate Diluent
• Micro ELISA Plate (Dismountable)
• Plate Sealer
• Product Description
• Reference Standard
• Reference Standard & Sample Diluent
• Stop Solution
• Substrate Reagent

Preparation and Storage

Background: GRB 14

Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific phosphotyrosine-containing receptor sequences. GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. An additional member of the GRB7 family, designated GRB14, contains a pleckstrin homology domain in its central region and a carboxy terminal SH2 domain.

Additional GRB 14 Products

• All Products for GRB 14
• GRB 14 ELISA Kits
• GRB 14 Lysates
• GRB 14 Primary Antibodies
• GRB 14 Proteins and Enzymes

Product Documents for Human GRB 14 ELISA Kit (Colorimetric)