Recombinant Human GRP78/HSPA5 His Protein

Novus Biologicals | Catalog # NBP2-23104

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all GRP78/HSPA5 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 19-654 of Human GRP78/HSPA5

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEEEDKKE DVGTVVGIDL GTTYSCVGVF KNGRVEIIAN DQGNRITPSY VAFTPEGERL IGDAAKNQLT SNPENTVFDA KRLIGRTWND PSVQQDIKFL PFKVVEKKTK PYIQVDIGGG QTKTFAPEEI SAMVLTKMKE TAEAYLGKKV THAVVTVPAY FNDAQRQATK DAGTIAGLNV MRIINEPTAA AIAYGLDKRE GEKNILVFDL GGGTFDVSLL TIDNGVFEVV ATNGDTHLGG EDFDQRVMEH FIKLYKKKTG KDVRKDNRAV QKLRREVEKA KRALSSQHQA RIEIESFYEG EDFSETLTRA KFEELNMDLF RSTMKPVQKV LEDSDLKKSD IDEIVLVGGS TRIPKIQQLV KEFFNGKEPS RGINPDEAVA YGAAVQAGVL SGDQDTGDLV LLDVCPLTLG IETVGGVMTK LIPRNTVVPT KKSQIFSTAS DNQPTVTIKV YEGERPLTKD NHLLGTFDLT GIPPAPRGVP QIEVTFEIDV NGILRVTAED KGTGNKNKIT ITNDQNRLTP EEIERMVNDA EKFAEEDKKL KERIDTRNEL ESYAYSLKNQ IGDKEKLGGK LSSEDKETME KAVEEKIEWL ESHQDADIED FKAKKKELEE IVQPIISKLY GSAGPPPTGE EDTAEKDEL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

72.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GRP78/HSPA5 His Protein

SDS-PAGE: Recombinant Human GRP78/HSPA5 His Protein [NBP2-23104]

SDS-PAGE: Recombinant Human GRP78/HSPA5 His Protein [NBP2-23104]

SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBP2-23104] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP2-23104
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

HSPA5 is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. Recombinant human HSPA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for Recombinant Human GRP78/HSPA5 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human GRP78/HSPA5 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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