GSK-3 alpha/beta [p Ser9] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-741

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all GSK-3 alpha/beta Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding GSK-3 alpha/beta conjugated to KLH. Accession # P18266

Modification

p Ser9

Specificity

Specific for the ~46 kDa GSK3 beta protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3beta band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 alpha/beta [p Ser9] Antibody - Azide Free

Western Blot: GSK-3 alpha/beta [p Ser9] Antibody [NB300-741]

Western Blot: GSK-3 alpha/beta [p Ser9] Antibody [NB300-741]

Western Blot: GSK3 alpha/beta [p Ser9] Antibody [NB300-741] - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Applications for GSK-3 alpha/beta [p Ser9] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha/beta

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3a and GSK3b. Specifically, GSK3b has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3b by insulin (Sutherland et al., 1993).

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

Entrez Gene IDs

2931 (Human); 606496 (Mouse); 50686 (Rat)

Gene Symbol

GSK3A

UniProt

P18266 (Rat)

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta [p Ser9] Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GSK-3 alpha/beta [p Ser9] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for GSK-3 alpha/beta [p Ser9] Antibody - Azide Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Has NB300-741 been validated on Drosophila melanogaster?

    A: This has not yet been validated on Drosophila samples. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

  • Q: Is the immunogen derived from GSK3 beta or GSK3 alpha? If it is derived from GSK3 beta, is the gene GSK3A? Or the gene name should be GSK3B?

    A: Our GSK-3 alpha/beta (pSer9) antibody with catalogue number NB300-741 was raised against a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 residue of human GSK3 beta. The antibody is specific for the ~46kDa GSK3 beta protein phosphorylated at Ser9, but it also weakly labels the ~51kDa GSK3 alpha protein due to the high degree of homology between the two isoforms.

  • Q: Has NB300-741 been validated on Drosophila melanogaster?

    A: This has not yet been validated on Drosophila samples. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

  • Q: Is the immunogen derived from GSK3 beta or GSK3 alpha? If it is derived from GSK3 beta, is the gene GSK3A? Or the gene name should be GSK3B?

    A: Our GSK-3 alpha/beta (pSer9) antibody with catalogue number NB300-741 was raised against a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 residue of human GSK3 beta. The antibody is specific for the ~46kDa GSK3 beta protein phosphorylated at Ser9, but it also weakly labels the ~51kDa GSK3 alpha protein due to the high degree of homology between the two isoforms.

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