Q: Your website shows two different products for GSTA1protein. The references are: - GSTA1 Protein NBP1-30307 -0.1mg; - GSTA1 Protein P3495-0.1mg. What are the biological differences between these two products that explain such a difference in price? I wish to conduct studies of enzyme activity, so I need a functional enzyme, which one should I use?

A: Either product would work as both have been validated for use in functional studies. While both products are very similar we license them from different labs therefore they are different prices.

Recombinant Human GSTA1 Protein

Novus Biologicals | Catalog # NBP1-30307

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

View all GSTA1 Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-222 of Human GSTA1

Source: E.coli

Amino Acid Sequence: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

25.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 35,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GSTA1 Protein

SDS-PAGE: Recombinant Human GSTA1 Protein [NBP1-30307]

SDS-Page: GSTA1 Protein [NBP1-30307] - GSTA1, 25.6 kDa (222aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation, and Storage

NBP1-30307

Formulation	20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSTA1

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Glutathione S-transferase A1

Alternate Names

EC 1.11.1.-, EC 2.5.1.18, EC 5.3.3.-, GST HA subunit 1, GST-epsilon, GSTA1-1

Gene Symbol

GSTA1

Additional GSTA1 Products

Product Documents for Recombinant Human GSTA1 Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human GSTA1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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