HA Tag Alexa Fluor® 594-conjugated Antibody

Recombinant Monoclonal Antibody.
  
  • Specificity
    Stains N-terminal,C-terminal, and Internal Sequence HA-tagged protein transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Recombinant Monoclonal Mouse IgG1 Clone # 912426
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    HA Tag peptide
    Accession # ABB51961
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of HA Tag in HEK293 Human Cell Line Transfected withHA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) HA-tagged proteins (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Mouse Anti-HA Tag Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC6875T). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HA Tag
  • Entrez Gene IDs:
    ABB51961.1 (Viral)
  • Alternate Names:
    HA Epitope Tag; HA Tag; HA; Hemagglutinin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 594-conjugated Antibody

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