HA Tag Antibody

Recombinant Monoclonal Antibody.
  
  • Specificity
    Detects N-terminal,C-terminal, and Internal Sequence HA-tagged proteins in Western blot.
  • Source
    Recombinant Monoclonal Mouse IgG1 Clone # 912426
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    HA Tag peptide
    Accession # ABB51961
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of HA-taggedproteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged
LGI-2, Internal Sequence HA-tagged CIGALT, and C-terminal HA-tagged FBLN. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-HA Peptide Monoclonal Antibody (Catalog # MAB6875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HA-tagged proteins at approximately 60, 37, and 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
HA Tag in HEK293 Human Cell Line. HA Tag was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-HA Tag Monoclonal Antibody (Catalog # MAB6875) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of HA in HEK293 Human Cell Line Transfected with HA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with HA-tagged proteins was stained with Mouse Anti-HA Monoclonal Antibody (Catalog # MAB6875, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HA Tag
  • Entrez Gene IDs:
    ABB51961.1 (Viral)
  • Alternate Names:
    HA Epitope Tag; HA Tag; HA; Hemagglutinin
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