Detection of HA-taggedproteins by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged LGI-2, Internal Sequence HA-tagged CIGALT, and C-terminal HA-tagged FBLN. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-HA Peptide Monoclonal Antibody (Catalog # MAB6875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HA-tagged proteins at approximately 60, 37, and 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HA Tag in HEK293 Human Cell Line.
HA Tag was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-HA Tag Monoclonal Antibody (Catalog # MAB6875) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of HA in HEK293 Human Cell Line Transfected with HA-tagged Proteins by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with HA-tagged proteins was stained with Mouse Anti-HA Monoclonal Antibody (Catalog # MAB6875, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.