HA Tag Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC6875R-025
IC6875R-100
Detection  of HA Tag in HEK293 Human Cell Line Transfected withHA-tagged Proteins by Flow Cytometry.
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HA Tag Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Stains N-terminal,C-terminal, and Internal Sequence HA-tagged protein transfectants but not irrelevant transfectants in flow cytometry.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 912426
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HA tag peptide
Accession # ABB51961
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of HA Tag in HEK293 Human Cell Line Transfected withHA-tagged Proteins by Flow Cytometry. View Larger

Detection of HA Tag in HEK293 Human Cell Line Transfected withHA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) HA-tagged proteins (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Mouse Anti-HATag Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC6875R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HA Tag

Entrez Gene IDs
ABB51961.1 (Viral)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

Product Datasheets

FAQs

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