HDGF Antibody (5U7A7)

Novus Biologicals | Catalog # NBP3-15281

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5U7A7 expressed in HEK293
View all HDGF Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HDGF (P51858). YQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNST

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDGF Antibody (5U7A7)

Western Blot: HDGF Antibody (5U7A7) [NBP3-15281]

Western Blot: HDGF Antibody (5U7A7) [NBP3-15281]

Western Blot: HDGF Antibody (5U7A7) [NBP3-15281] - Western blot analysis of extracts of various cell lines, using HDGF antibody (NBP3-15281) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281]

Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281]

Immunocytochemistry/Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] - Immunofluorescence analysis of U2OS cells using HDGF Rabbit mAb (NBP3-15281) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281]

Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281]

Immunocytochemistry/Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] - Immunofluorescence analysis of NIH/3T3 cells using HDGF Rabbit mAb (NBP3-15281) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
HDGF Antibody (5U7A7)

Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] -

Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] - Confocal imaging of paraffin-embedded Human colon tissue using HDGF Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
HDGF Antibody (5U7A7)

Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] -

Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] - Confocal imaging of paraffin-embedded Mouse colon tissue using HDGF Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
HDGF Antibody (5U7A7)

Western Blot: HDGF Antibody (5U7A7) [NBP3-15281] -

Western Blot: HDGF Antibody (5U7A7) [NBP3-15281] - Western blot analysis of various lysates, using HDGF Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for HDGF Antibody (5U7A7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HDGF

The HDGF gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic andDNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thoughtinitially to be located on chromosom

Long Name

Hepatoma-derived Growth Factor

Alternate Names

HuHGF

Gene Symbol

HDGF

Additional HDGF Products

Product Documents for HDGF Antibody (5U7A7)

Certificate of Analysis

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Product Specific Notices for HDGF Antibody (5U7A7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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