Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP1-92010PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
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Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human HS6ST1.

Source: E. coli

Amino Acid Sequence: QRYQYKRQLERREQRLRSREERLLHRAKEALPREDADEPGRVPTEDYMSHIIEKW

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-92010.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP1-92010PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1

HS6ST1 (Heparan-sulphate 6-O-sulfotransferase 1) is a single-pass type II transmembrane protein that catalyses the transfer of sulphate from PAPS (3'-phosphoadenosine 5'-phosphosulphate) to position 6 of the N-sulphoglucosamine residue of heparan sulphate. It is expressed in foetal brain and liver and is thought to play a significant role in axonal guidance through sulphation of heparan sulphate proteoglycans, with HS6ST1 expression mirroring that of Slit1 and Slit2.

Alternate Names

DKFZp547H098, EC 2.8.2, EC 2.8.2.-, FLJ25392, heparan sulfate 6-O-sulfotransferase 1, heparan-sulfate 6-sulfotransferase, HS6ST-1, HS6STheparan-sulfate 6-O-sulfotransferase 1, MGC116899, MGC116901

Gene Symbol

HS6ST1

Additional Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail